BiasAway: a tool to generate nucleotide composition-matched DNA sequences

Upload your FASTA file(s)

You're running BiasAway v3.3.0 with Synthetic k-mer shuffling

Note: The input file should contain DNA sequences provided using the FASTA format. For large fasta files, we recommend to use compressed (.gz) files. Do not upload word processor or office software documents containing sequence data: instead, make sure that such data is stored in a plain text file.

Foreground file FASTA format:
How many background sequences per each foreground sequence will be generated?
K-mer used for the shuffling:
Seed number to initialize the random number generator:
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What is BiasAway?

The BiasAway software tool is introduced to generate nucleotide composition-matched DNA sequences. It is available as open source code from bitbucket.

The tool provides user with six approaches to generate synthetic or genomic background sequences matching mono- and dinucleotide composition of user-provided foreground sequences:

synthetic k-mer shuffled sequences
synthetic k-mer shuffled sequences in a sliding window
genomic mononucleotide distribution matched sequences
genomic mononucleotide distribution within a sliding window matched sequences

BiasAway module: Synthetic k-mer shuffling (k)

Upload your FASTA file(s)

What is BiasAway?